nickel column Search Results


nickel columns  (Qiagen)
95
Qiagen nickel columns
Nickel Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel-nitrilotriacetic acid gravity flow affinity column  (Fisher Scientific)
90
Fisher Scientific nickel-nitrilotriacetic acid gravity flow affinity column
Nickel Nitrilotriacetic Acid Gravity Flow Affinity Column, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel column beyogold his-tag purification resin  (Beyotime)
90
Beyotime nickel column beyogold his-tag purification resin
Nickel Column Beyogold His Tag Purification Resin, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel beads column (ni 2+ -nta  (Qiagen)
90
Qiagen nickel beads column (ni 2+ -nta
Nickel Beads Column (Ni 2+ Nta, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel-nitrilotriacetic acid column  (Qiagen)
90
Qiagen nickel-nitrilotriacetic acid column
Nickel Nitrilotriacetic Acid Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel columns  (Becton Dickinson)
90
Becton Dickinson nickel columns
Nickel Columns, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel-chelating sepharose column  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd nickel-chelating sepharose column
Nickel Chelating Sepharose Column, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel-affinity chromatography column  (Yeasen Biotechnology)
90
Yeasen Biotechnology nickel-affinity chromatography column
Nickel Affinity Chromatography Column, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel–nitrilotriacetic acid agarose column  (Promega)
90
Promega nickel–nitrilotriacetic acid agarose column
Nickel–Nitrilotriacetic Acid Agarose Column, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel column affinity chromatography  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd nickel column affinity chromatography
Characterisation of TomLOXF . A . The detection of his-tagged proteins was realized on Western blot with an anti-His-tag antibody (1 and 2) and we evaluated the purity of the protein through SDS-PAGE and Coomassie blue staining (3). 1: Page Ruler Plus Prestained Protein Ladder (Fermentas), 1: total proteins extracted from TomLOXF-expressing E. coli , 2 and 3: partially-purified protein extracted from TomLOXF-expressing clone by <t>nickel</t> <t>affinity</t> <t>chromatography.</t> B . LOX activity was evaluated on partially-purified recombinant TomLOXF with linoleic (C18:2) and linolenic (C18:3) acids as substrate. Reaction was performed at pH 6.0, room temperature. C . Linolenic and linoleic acids were both incubated with extracts of E. coli expressing TomLOXF in oxygenated buffer. Produced hydroperoxides were separated by HPLC, and the profile of compounds absorbing at 234 nm was compared with the profile of pure 13-HPOT, 13-HPOD, 9-HPOT and 9-HPOD.
Nickel Column Affinity Chromatography, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel-sepharose column  (Prochem Inc)
90
Prochem Inc nickel-sepharose column
Characterisation of TomLOXF . A . The detection of his-tagged proteins was realized on Western blot with an anti-His-tag antibody (1 and 2) and we evaluated the purity of the protein through SDS-PAGE and Coomassie blue staining (3). 1: Page Ruler Plus Prestained Protein Ladder (Fermentas), 1: total proteins extracted from TomLOXF-expressing E. coli , 2 and 3: partially-purified protein extracted from TomLOXF-expressing clone by <t>nickel</t> <t>affinity</t> <t>chromatography.</t> B . LOX activity was evaluated on partially-purified recombinant TomLOXF with linoleic (C18:2) and linolenic (C18:3) acids as substrate. Reaction was performed at pH 6.0, room temperature. C . Linolenic and linoleic acids were both incubated with extracts of E. coli expressing TomLOXF in oxygenated buffer. Produced hydroperoxides were separated by HPLC, and the profile of compounds absorbing at 234 nm was compared with the profile of pure 13-HPOT, 13-HPOD, 9-HPOT and 9-HPOD.
Nickel Sepharose Column, supplied by Prochem Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel talon affinity column  (Qiagen)
90
Qiagen nickel talon affinity column
Characterisation of TomLOXF . A . The detection of his-tagged proteins was realized on Western blot with an anti-His-tag antibody (1 and 2) and we evaluated the purity of the protein through SDS-PAGE and Coomassie blue staining (3). 1: Page Ruler Plus Prestained Protein Ladder (Fermentas), 1: total proteins extracted from TomLOXF-expressing E. coli , 2 and 3: partially-purified protein extracted from TomLOXF-expressing clone by <t>nickel</t> <t>affinity</t> <t>chromatography.</t> B . LOX activity was evaluated on partially-purified recombinant TomLOXF with linoleic (C18:2) and linolenic (C18:3) acids as substrate. Reaction was performed at pH 6.0, room temperature. C . Linolenic and linoleic acids were both incubated with extracts of E. coli expressing TomLOXF in oxygenated buffer. Produced hydroperoxides were separated by HPLC, and the profile of compounds absorbing at 234 nm was compared with the profile of pure 13-HPOT, 13-HPOD, 9-HPOT and 9-HPOD.
Nickel Talon Affinity Column, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Characterisation of TomLOXF . A . The detection of his-tagged proteins was realized on Western blot with an anti-His-tag antibody (1 and 2) and we evaluated the purity of the protein through SDS-PAGE and Coomassie blue staining (3). 1: Page Ruler Plus Prestained Protein Ladder (Fermentas), 1: total proteins extracted from TomLOXF-expressing E. coli , 2 and 3: partially-purified protein extracted from TomLOXF-expressing clone by nickel affinity chromatography. B . LOX activity was evaluated on partially-purified recombinant TomLOXF with linoleic (C18:2) and linolenic (C18:3) acids as substrate. Reaction was performed at pH 6.0, room temperature. C . Linolenic and linoleic acids were both incubated with extracts of E. coli expressing TomLOXF in oxygenated buffer. Produced hydroperoxides were separated by HPLC, and the profile of compounds absorbing at 234 nm was compared with the profile of pure 13-HPOT, 13-HPOD, 9-HPOT and 9-HPOD.

Journal: BMC Plant Biology

Article Title: The elicitation of a systemic resistance by Pseudomonas putida BTP1 in tomato involves the stimulation of two lipoxygenase isoforms

doi: 10.1186/1471-2229-11-29

Figure Lengend Snippet: Characterisation of TomLOXF . A . The detection of his-tagged proteins was realized on Western blot with an anti-His-tag antibody (1 and 2) and we evaluated the purity of the protein through SDS-PAGE and Coomassie blue staining (3). 1: Page Ruler Plus Prestained Protein Ladder (Fermentas), 1: total proteins extracted from TomLOXF-expressing E. coli , 2 and 3: partially-purified protein extracted from TomLOXF-expressing clone by nickel affinity chromatography. B . LOX activity was evaluated on partially-purified recombinant TomLOXF with linoleic (C18:2) and linolenic (C18:3) acids as substrate. Reaction was performed at pH 6.0, room temperature. C . Linolenic and linoleic acids were both incubated with extracts of E. coli expressing TomLOXF in oxygenated buffer. Produced hydroperoxides were separated by HPLC, and the profile of compounds absorbing at 234 nm was compared with the profile of pure 13-HPOT, 13-HPOD, 9-HPOT and 9-HPOD.

Article Snippet: His-tagged proteins were purified by nickel column affinity chromatography (Äkta, Amersham Pharmacia Biotech) according the method described in Boutaud and Brash [ ].

Techniques: Western Blot, SDS Page, Staining, Expressing, Purification, Affinity Chromatography, Activity Assay, Recombinant, Incubation, Produced